FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Carrions, Chrysomya albiceps, cypermethrin, forensic entomology, post mortem interval
This research work assessed effects of varying volumes of cypermethrin pesticide on the larval body length, weight, and developmental time of blowfly Chrysomya albiceps (Diptera: Calliphoridae) reared on rabbit carrions. Sixteen rabbits (Oryctolagus cuniculus) with mean weight 2.14±0.18 kg (Mean ± S.D.) were sacrificed in groups of four with 3, 6 and 12 ml, respectively of cypermethrin pesticide. The control group (no cypermethrin pesticide) was sacrificed by cervical dislocation. These dosages are the same with those that are normally observed in homicide and abusive cases involving cypermethrin pesticide injection doses. Mettler Toledo weighing balance (with sensitivity of 0.001-1 g) was used to record the weights of larvae and a pair of compasses was used to measure the body length and read with a transparent meter rule. All stages of the insect were monitored and observations recorded. The mean minimum lengths and weights, i.e. 0.0045 g: 03.95 mm for 12 ml; 0.0053 g: 05.77 mm for 6 ml, and 0.0074 g: 06.56 mm for 3 ml were attained at 108 h after eggs were laid compared to the control group that recorded 0.0123 g: 11.13 mm at the same time. The mean total developmental periods were reached at 450.22 h (18.76 days), 401.05 h (16.71 days), 380.28 h (15.85 days) and 281.24 h (11.42 days) respectively for 12 ml, 6 ml, 3 ml groups, and the control. This result showed the possible post mortem interval estimation error of 7.34 days, 5.29 days and 4.43 days respectively in cases where 12, 6 and 3 ml dosages of cypermethrin pesticide are involved compared with the control group. Absolute care must be employed in the calculation, interpretation and usage of insects’ data in forensic entomology, when and where toxins and drugs may be involved in the investigation.
Abdalla SB & Mohamed AR 2014. Morphological and taxonomical characteristics of Chrysomya putoria (Dipteria: Calliphoridae). Sud. J. Sci., 6(1): 35-40. Adams ZJ & Halls MJ 2003. Methods used in the killing and preservation of blowfly larvae and Their effects on the PMI length. Foren. Sci. Int., 138: 50-61. Aggarwal P, Jamshed N, Ekka M & Imran A 2015. Suicidal poisoning with cypermethrin: A clinical dilemma in the emergency department. J. Emerg Trauma Shock, 8(2): 123–125. Beyer JC, Enos WF & Stajic K 1980. Drugs identification through the analysis of maggots. J. Foren. Sci., 25: 411-412. Bourel B, Hedouim V, Martin-Bouyer L, Becart G, Deveaux M & Gosset D 1999. Necrophilous insects’ succession on rabbit carrion in sand dune habitats in Northern France. J. Med. Entom., 36: 420-425. Carson R 2002. Silent Spring, Anni. Edn Houghton Mifflin Publishing Company, USA, pp. 16-28. Carvalho LM 2010. Toxicology and forensic entomology. In: Amendt J, Campobasso CP, Goff ML & Grassberger M (eds), Current Concepts in Forensic Entomology, pp. 163-178. Springer: Heidelberg, Germany. Catts EP & Goff ML 1992. Forensic entomology in criminal investigation. Ann. Rev. Entom. 37: 253-272. Cox C 1996. Insecticide fact sheet: Cypermethrin. J. Pest Ref., 16: 15- 20. Dayananda R & Kiran J 2013. Entomotoxicology. Int. J. Med. Toxic. Foren. Med., 3(2):b71-74. Egborge ABM 1994. Water pollution in Nigeria:Biodiversity and chemistry of Warri River. Ben Miller Books Nigeria Limited, Warri, Nigeria, pp. 275-288. Ekrem E., Aylin E., Fevzi U & David BR 2007. Effects of Cypermethrin exposed hosts on egg-adult development time, numbers of offspring, sex ratio, longevity, and size of Apanteles galleriae Wilkinson (Hymenptera: Braconidae). Belgian J. Zoo., 137(1): 27-31. Gautam L, Pathak R, Danlami HS & Cole MD 2013. Entomotoxicology: Alternative matrices for forensic toxicology. Inter. J. Med. Toxic. Foren. Med., 3(2). www.forensicmag.com. Goff ML & Lord WD 1994. Entomotoxicology: A new area of forensic investigation. Amer. J. Foren. Medi. Path., 15: 51-57. Goff ML, Brown WA, Hewadi-Karana KA & Omori AI 1991. Effect of heroin in the decomposing tissue on the development of Boettecherisca peregrine (Diptera: Sarcophagidae) and the implications of the effect on the estimation of the post-mortem interval using arthropod development patterns. J. Foren. Sci., 36: 537-542. Goff ML, Omori AI & Godbrod JR 1989. Effect of cocaine in tissues on the development rate of Boettcherisca peregrine (Diptera: Sarcophagidae). J. Med. Entomo., 26: 91-93. Introna F, Campobasso CP & Goff ML 2001. Entomotoxicology. For. Sci. Int., 120: 42–47. Jodie K, Margaret S& Williams S 2006. Alternative to Animal Testing and Experiment (AATEX) 14, Special Issue, Proc. 6th World Congress on Alternative and Animal use in Sciences, August 21-25, Tokyo, Japan, pp. 163-165. Kharbonche H, Augsburger M, Cherix D, Sportkert F, Giround C, Wyss C, Champed D & Mangne P 2008. Codeine accumulation and elimination in larvae, pupae, and stages of the Blowfly L.sericata and its effects on its development. Int. J. Leg. Medic, 122: 205-211. Krespi L, Rabasse JM, Dedryver CA & Nenon JP 1991. Effect of three insecticides on the life cycle of Aphidiusuzbekistanicus Luz. (Hym., Aphidiidae). J. Appl. Entomol., 111:113-119. Liu MY, Bull DL, & Plapp FW 1998. Effects of exposure to cypermethrin on saxitoxin binding in susceptible and pyrethroid resistant houseflies. Insect Biochem. Physiol., 37: 73-79. McGavin GC 2007. Expedition Field Techniques: Insects and other Arthropods. The centre for supporting field research, exploration and outdoor learning.Royal Geographical Society with IBG, 1 Kensington Gore, London. O’Brien C & Turner B 2004. Impact of paracetamol on Calliphora vicina larval development. Int. J. Legal Med., 118: 188-189. Queiroz CMM, Mello PR & Lima MM 1997. Morphological aspects of the larval instar of Chrysomya albiceps (Dipteria, Calliphoridae) reared in the laboratory. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, 92(2): c187-196. Rivers DB & Dahlem GA 2014. The science of forensic entomology. Wiley-Blackwell, New York. 392pp. Shukla Y, Yadav A & Arora A 2002. Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin. Cancer Letters, 182: 33-41. Smilanick JM., Zalom FG & Ehler LE 1996. Effect of metamidophos residue on the pentatomid egg parasitoids Trisolcus basalis and T. utahensis (Hym.: Scelionidae). Biol. Control, 6: 193-201. Suh CPC, Orr DB & Van Duyn JW 2000. Effect of insecticideson Trichogramma exiguum (Hymenoptera; Trichogrammatidae) preimaginal development and adult survival. J. Econ. Entomol., 93: 577-583. Tracqui A., Keyseri-Tracqui C., Kintz P& Lides B 2004. Entomotoxicology for the forensic toxicologists: Much ado about nothing. Int. J. Legal Medi., 118: 194-196. Willrich MM& Boethel DJ 2001. Effects of diflubenzuronon Pseudoplusia includens (Lepidoptera: Noctuidae) and its parasitoid Copidosoma floridanum (Hymenoptera: Encyrtidae). Environ. Entomol., 30: 794-797. Xu JSA & Cheng X 2001. Variation in the susceptibility of Diadegma insulare (Hymenoptera: Ichneumonidae) to permethrin. J. Eco. Entomol., 94: 541-546.
